Escherichia coli RuvC polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Detection of RuvC (19kD) proteins in the cell extracts of E. coli carrying the plasmids which encode the indicated Mutant RuvC proteins by Western blotting using RuvC polyclonal antibody (Cat # PAB15571).
WT : wild type RuvC protein.
vector : vector plasmid.
D7G, D7N, P8L : mutant RuvC proteins.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant Escherichia coli RuvC.
Immunogen
Recombinant protein corresponding to full length Escherichia coli RuvC.
Host
Rabbit
Reactivity
Escherichia coli
Form
Liquid
Recommend Usage
Western Blot (1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Detection of RuvC (19kD) proteins in the cell extracts of E. coli carrying the plasmids which encode the indicated Mutant RuvC proteins by Western blotting using RuvC polyclonal antibody (Cat # PAB15571).
WT : wild type RuvC protein.
vector : vector plasmid.
D7G, D7N, P8L : mutant RuvC proteins. -
Gene Info — ruvC
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Publication Reference
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Mutational analysis on structure-function relationship of a holliday junction specific endonuclease RuvC.
Ichiyanagi K, Iwasaki H, Hishida T, Shinagawa H.
Genes to Cells 1998 Sep; 3(9):575.
Application：WB-Tr, E. coli, Strain HRS1200.
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Processing the holliday junction in homologous recombination.
Shinagawa H, Iwasaki H.
Trends in Biochemical Sciences 1996 Mar; 21(3):107.
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Identification of four acidic amino acids that constitute the catalytic center of the RuvC Holliday junction resolvase.
Saito A, Iwasaki H, Ariyoshi M, Morikawa K, Shinagawa H.
PNAS 1995 Aug; 92(16):7470.
Application：WB-Re, Recombinant protein.
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Mutational analysis on structure-function relationship of a holliday junction specific endonuclease RuvC.
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