FOS polyclonal antibody
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Immunohistochemistry
Immunohistochemical staining of rat brain. c-Fos was induced in the hypothalamic arcuate nucleus of an adult male Wistar rat with i.p. injection of N-methyl-D-aspartate. The brain was fixed by transcardiac perfusion of the rat with formaldehyde (4%). cFos immunoreactivity was detected on floated cryo-sections of the hypothalamic arcuate nucleus with FOS polyclonal antibody (Cat# PAB14342) (1:5000) using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.
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Specification
Product Description
Sheep polyclonal antibody raised against synthetic peptide of FOS.
Immunogen
A synthetic peptide (conjugated with diphtheria toxoid) corresponding to amino acids 2-17 of FOS.
Sequence
MFSGFNADYEASSSRC
Host
Sheep
Reactivity
Cat, Hamster, Human, Mouse, Rabbit, Rat
Specificity
This antiserum shows a high level of specificity for cFOS confirmed by immunohostochemistry.
Form
Lyophilized
Purification
Affinity purification
Recommend Usage
Immunohistochemistry
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS.
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with 50 uL of sterile water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry
Immunohistochemical staining of rat brain. c-Fos was induced in the hypothalamic arcuate nucleus of an adult male Wistar rat with i.p. injection of N-methyl-D-aspartate. The brain was fixed by transcardiac perfusion of the rat with formaldehyde (4%). cFos immunoreactivity was detected on floated cryo-sections of the hypothalamic arcuate nucleus with FOS polyclonal antibody (Cat# PAB14342) (1:5000) using the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary. -
Publication Reference
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Disinhibition of the rostral ventral medulla increases blood pressure and Fos expression in bulbospinal neurons.
Minson JB, Llewellyn-Smith IJ, Arnolda LF, Pilowsky PM, Oliver JR, Chalmers JP.
Brain Research 1994 May; 646(1):44.
Application：IHC, Rat, Brainstem.
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Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein.
van Straaten F, Muller R, Curran T, Van Beveren C, Verma IM.
PNAS 1983 Jun; 80(11):3183.
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Disinhibition of the rostral ventral medulla increases blood pressure and Fos expression in bulbospinal neurons.
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