Collagen Type IV polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against native Collagen Type IV.
Immunogen
Native purified Collagen Type IV from rodent tumour tissue.
Host
Rabbit
Reactivity
Mouse, Rat
Specificity
This antibody reacts with rat collagen type IV 100%; rat collagen type I, II, III and V <0.1%; rat fibronectin <0.1%; murine laminin <0.1% in RIA at 1:5000 dilution.
Form
lyophilized
Recommend Usage
ELISA (1:200)
Immunohistochemistry (1:500)
Immunofluorescence (1:80)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS.
Storage Instruction
Store at 4°C on dry atmosphere. For prolonged storage prepare appropriate aliquots and store at or below -20°C.
After reconstitution with sterile distilled water, aliqout and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Radioimmunoassay
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