MKI67 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MKI67.
Immunogen
A synthetic peptide corresponding to human MKI67.
Host
Rabbit
Theoretical MW (kDa)
67
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~67 KDa of MKI67.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (0.1-1 ug/mL)
Immunohistochemistry (2-5 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Western Blot (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (Antibody Stabilizer).
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human lymph node stained with MKI67 polyclonal antibody (Cat # PAB12698) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MKI67
Entrez GeneID
4288Gene Name
MKI67
Gene Alias
KIA, Ki-67
Gene Description
antigen identified by monoclonal antibody Ki-67
Omim ID
176741Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq
Other Designations
OTTHUMP00000020739|proliferation-related Ki-67 antigen
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