Goat Anti-Rabbit IgG F(c) secondary antibody (Rhodamine)
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Specification
Product Description
Goat anti-rabbit IgG recognizes rabbit IgG F(c) fragment. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with Rhodamine.
Immunogen
Rabbit IgG F(c) fragment
Host
Goat
Reactivity
Rabbit
Form
Lyophilized
Conjugation
Rhodamine (TRITC)
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation Note
FP Value: 2.9 moles Rhodamine (TRITC) per mole of IgG
Recommend Usage
FLISA (1:10000-1:50000)
Flow Cytometry (1:500-1:2500)
Immunofluorescence (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (10 mg/mL BSA (Immunoglobulin and Protease free), 0.01% (w/v) sodium azide).
Storage Instruction
Store at 4°C prior to restoration.
After reconstitution with 1.0 mL deionized water (or equivalent), store at -20°C or below.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Flow Cytometry
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Publication Reference
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Heat shock protein 70 as a supplementary receptor facilitates enterovirus 71infections in vitro.
Xu T, Lin Z, Wang C, Li Y, Xia Y, Zhao M, Hua L, Chen Y, Guo M, Zhu B.
Microbial Pathogenesis 2018 Dec; 128:106.
Application：IP, Human, RD cells.
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Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies.
J A Titus, R Haugland, S O Sharrow, D M Segal.
Journal of Immunological Methods 1982 Apr; 50(2):193.
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Heat shock protein 70 as a supplementary receptor facilitates enterovirus 71infections in vitro.
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