HA tag polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
HA tag polyclonal antibody (Cat # PAB10343) detects HA-tagged recombinant proteins by western blot.
HA-tag polyclonal antibody at a 1 : 2,000 dilution, was used to detect 1.0 ug of 12-Epitope Tag Protein Marker Lysate containing the HA epitope tag.
A 4-20% gradient gel was used to resolve the protein by SDS-PAGE.
The lysate was transferred to nitrocellulose using standard methods.
After blocking, the membrane was probed with HA-tag polyclonal antibody for 1 h at room temperature followed by washes and reaction with a 1 : 20,000 dilution of IRDye™ 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 30 min at room temperature.
LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining on formalin fixed paraffin embedded mouse aorta tissue with HA tag polyclonal antibody (Cat # PAB10343) at 1 : 2000 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptides of HA (Hemagglutinin) tag.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to YPYDVPDYA of HA Tag.
Host
Rabbit
Specificity
This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. In western blotting of bacterial extracts, This antibody does not cross-react with endogenous proteins.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:20000-1:150000)
Western Blot (1:2000-1:10000)
Immunohistochemistry (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
HA tag polyclonal antibody (Cat # PAB10343) detects HA-tagged recombinant proteins by western blot.
HA-tag polyclonal antibody at a 1 : 2,000 dilution, was used to detect 1.0 ug of 12-Epitope Tag Protein Marker Lysate containing the HA epitope tag.
A 4-20% gradient gel was used to resolve the protein by SDS-PAGE.
The lysate was transferred to nitrocellulose using standard methods.
After blocking, the membrane was probed with HA-tag polyclonal antibody for 1 h at room temperature followed by washes and reaction with a 1 : 20,000 dilution of IRDye™ 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 30 min at room temperature.
LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining on formalin fixed paraffin embedded mouse aorta tissue with HA tag polyclonal antibody (Cat # PAB10343) at 1 : 2000 dilution.Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Dephosphorylation of NFAT by Calcineurin inhibits Skp2-mediated degradation.
Shunsuke Hanaki, Makoto Habara, Yuki Sato, Haruki Tomiyasu, Yosei Miki, Shusaku Shibutani, Midori Shimada.
Journal of Biochemistry 2023 Nov; [Epub]:0.
Application：WB, Human, HEK293 cells.
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Dephosphorylation of the EGFR protein by calcineurin at serine 1046/1047 enhances its stability.
Takahiro Masaki, Makoto Habara, Shusaku Shibutani, Shunsuke Hanaki, Yuki Sato, Haruki Tomiyasu, Midori Shimada.
Biochemical and Biophysical Research Communications 2023 Jan; 641:84.
Application：IP, Human, HEK 293 cells.
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Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.
Field J, Nikawa J, Broek D, MacDonald B, Rodgers L, Wilson IA, Lerner RA, Wigler M.
Molecular and Cellular Biology 1988 May; 8(5):2159.
Application：AFC, IP, WB-Re, Recombinant proteins.
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Dephosphorylation of NFAT by Calcineurin inhibits Skp2-mediated degradation.
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