MARK2 (phospho T595) polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot using MARK2 (phospho T595) polyclonal antibody (Cat # PAB10332) shows detection of a band at ~82 kDa corresponding to phosphorylated MARK2 (arrowhead).
HEK293 cells were transfected with pCMV-3 x Flag-MARK2 plus pCMV-Flag-PKC-zeta (lane 3) or pCMV-3 x Flag-MARK2 only (lane 4).
Total cell lysates were run in a 4-12% Nupage SDS-gel and probed with MARK2 (phospho T595) polyclonal antibody (Cat # PAB10332).
An untransfected HEK293 cell lysate was used as a negative control (lane 2).
MW markers are shown for size comparison (lane 1).
PKC-zeta appears to be involved in the phosphorylation of T595 on MARK2 as increased amounts of phospho- specific staining are observed in lysates from cells transfected with both MARK2 and PKC-zeta.
Personnel Communication, S. Shaw, NCI, Center for Cancer Research.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MARK2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T595 of human MARK2.
Host
Rabbit
Reactivity
Bovine, Dog, Human, Mouse, Rat
Specificity
Reactivity occurs against human MARK2 pT595 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human MARK2 is minimal by ELISA. Expect at least partial reactivity with other isoforms of MARK. The immunogen sequence is present in all forms identified to date and localizes to T595 on MARK2, T587 on MARK3, T591 on MARK1 and T568 on MARK4.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:1000-1:3000)
Western Blot (1:250-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot using MARK2 (phospho T595) polyclonal antibody (Cat # PAB10332) shows detection of a band at ~82 kDa corresponding to phosphorylated MARK2 (arrowhead).
HEK293 cells were transfected with pCMV-3 x Flag-MARK2 plus pCMV-Flag-PKC-zeta (lane 3) or pCMV-3 x Flag-MARK2 only (lane 4).
Total cell lysates were run in a 4-12% Nupage SDS-gel and probed with MARK2 (phospho T595) polyclonal antibody (Cat # PAB10332).
An untransfected HEK293 cell lysate was used as a negative control (lane 2).
MW markers are shown for size comparison (lane 1).
PKC-zeta appears to be involved in the phosphorylation of T595 on MARK2 as increased amounts of phospho- specific staining are observed in lysates from cells transfected with both MARK2 and PKC-zeta.
Personnel Communication, S. Shaw, NCI, Center for Cancer Research.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MARK2
Entrez GeneID
2011Protein Accession#
Q7KZI7;NP_059672Gene Name
MARK2
Gene Alias
EMK1, MGC99619, PAR-1, Par1b
Gene Description
MAP/microtubule affinity-regulating kinase 2
Omim ID
600526Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
ELKL motif kinase 1|Ser/Thr protein kinase PAR-1B|protein-serine/threonine kinase|serine/threonine kinase
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Interactome
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Disease
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Publication Reference
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Splicing alterations in human renal allografts: detection of a new splice variant of protein kinase Par1/Emk1 whose expression is associated with an increase of inflammation in protocol biopsies of transplanted patients.
Hueso M, Beltran V, Moreso F, Ciriero E, Fulladosa X, Grinyo JM, Seron D, Navarro E.
Biochimica et Biophysica Acta 2004 May; 1689(1):58.
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Atypical PKC phosphorylates PAR-1 kinases to regulate localization and activity.
Hurov JB, Watkins JL, Piwnica-Worms H.
Current Biology 2004 Apr; 14(8):736.
Application：WB-Tr, Human, HeLa, HEK 293 cells.
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PAR-1 is a Dishevelled-associated kinase and a positive regulator of Wnt signalling.
Sun TQ, Lu B, Feng JJ, Reinhard C, Jan YN, Fantl WJ, Williams LT.
Nature Cell Biology 2001 Jul; 3(7):628.
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Splicing alterations in human renal allografts: detection of a new splice variant of protein kinase Par1/Emk1 whose expression is associated with an increase of inflammation in protocol biopsies of transplanted patients.
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