SIRP alpha V6 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Biotinylated Human SIRP alpha V6, His Tag at 1 ug/mL (100 uL/well) on the streptavidin precoated plate (5 ug/mL). Dose response curve for Human CD47, hFc Tag with the EC50 of 0.21/ ug/mL determined by ELISA.
SEC-HPLC
The purity of Biotinylated Human SIRP alpha V6 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Biotinylated Human SIRP alpha V6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human SIRP alpha V6 (P78324 variant 6, Glu31-Arg370(S105P)) partial recombinant protein with His-Avi tag at C-Terminus expressed in HEK293 cells.
Sequence
Glu31-Arg370(S105P)
Host
Human
Theoretical MW (kDa)
39.9
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 0.21 ug/mL, messured by ELISA at 1 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Biotinylated Human SIRP alpha V6 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Biotinylated Human SIRP alpha V6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Biotinylated Human SIRP alpha V6, His Tag at 1 ug/mL (100 uL/well) on the streptavidin precoated plate (5 ug/mL). Dose response curve for Human CD47, hFc Tag with the EC50 of 0.21/ ug/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — SIRPA
Entrez GeneID
140885Protein Accession#
P78324 variant 6Gene Name
SIRPA
Gene Alias
BIT, CD172A, MFR, MYD-1, P84, PTPNS1, SHPS-1, SHPS1, SIRP, SIRP-ALPHA-1, SIRPalpha, SIRPalpha2
Gene Description
signal-regulatory protein alpha
Omim ID
602461Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000030001|SHP substrate-1|brain-immunoglobulin-like molecule with tyrosine-based activation motifs|macrophage fusion receptor|myd-1 antigen|protein tyrosine phosphatase, non-receptor type substrate 1|signal regulatory protein, alpha type 1|signal
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