IL31RA (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-31, His Tag at 5 ug/mL (100 uL/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.15 ug/mL determined by ELISA.
Surface Plasmon Resonance
Human IL-31 RA, hFc Tag captured on Protein A chip, can bind Human IL-31, His Tag with an affinity constant of 1.5nM as determined in a SPR assay (Biacore T200).
SEC-HPLC
The purity of Human IL-31 RA-is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-31 RA-on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human IL31RA (Q8NI17-1, Ala20-Glu519) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Ala20-Glu519
Host
Human
Theoretical MW (kDa)
83.8
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 1.15 ug/mL, messured by ELISA at 5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human IL-31 RA-is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-31 RA-on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human IL-31, His Tag at 5 ug/mL (100 uL/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.15 ug/mL determined by ELISA.Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human IL-31 RA, hFc Tag captured on Protein A chip, can bind Human IL-31, His Tag with an affinity constant of 1.5nM as determined in a SPR assay (Biacore T200). -
Gene Info — IL31RA
Entrez GeneID
133396Protein Accession#
Q8NI17-1Gene Name
IL31RA
Gene Alias
CRL, CRL3, GLM-R, GLMR, GPL, IL-31RA, MGC125346, PRO21384
Gene Description
interleukin 31 receptor A
Omim ID
609510Gene Ontology
HyperlinkGene Summary
IL31RA is related to gp130 (IL6ST; MIM 600694), the common receptor subunit for IL6 (MIM 147620)-type cytokines. Oncostatin M receptor (OSMR; MIM 601743) and IL31RA form the heterodimeric receptor through which IL31 (MIM 609509) signals. Expression of IL31RA and OSMR mRNA is induced in activated monocytes, and both mRNAs are constitutively expressed in epithelial cells (Dillon et al., 2004 [PubMed 15184896]).[supplied by OMIM
Other Designations
GP130 like receptor|OTTHUMP00000122547|class I cytokine receptor|gp130-like monocyte receptor|soluble type I cytokine receptor CRL3
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Interactome
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Disease
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