CD34 (Human) Recombinant Protein
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Specification
Product Description
Human CD34 (P28906-1, Ser32-Thr290) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Ser32-Thr290
Host
Human
Theoretical MW (kDa)
28.6
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 19.9 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CD34 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD34 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD34, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-CD34 Antibody, hFc Tag with the EC50 of 19.9 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — CD34
Entrez GeneID
947Protein Accession#
P28906-1Gene Name
CD34
Gene Alias
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Gene Description
CD34 molecule
Omim ID
142230Gene Ontology
HyperlinkGene Summary
CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 kD that is selectively expressed on human hematopoietic progenitor cells.[supplied by OMIM
Other Designations
CD34 antigen|OTTHUMP00000034733|OTTHUMP00000034734|hematopoietic progenitor cell antigen CD34
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Interactome
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