IL12RB1 (Human) Recombinant Protein
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More Files
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Surface Plasmon Resonance
Human IL-12B, His Tag immobilized on CM5 Chip can bind Human IL-12 R beta 1, His Tag with an affinity constant of 98.1nM as determined in a SPR assay (Biacore T20
SEC-HPLC
The purity of Human IL-12 R beta 1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-12 R beta 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human IL12RB1 (P42701-1, Cys24-Glu540) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Cys24-Glu540
Host
Human
Theoretical MW (kDa)
58.2
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The affinity constant of 98.1 nM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human IL-12 R beta 1 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human IL-12 R beta 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human IL-12B, His Tag immobilized on CM5 Chip can bind Human IL-12 R beta 1, His Tag with an affinity constant of 98.1nM as determined in a SPR assay (Biacore T20 -
Gene Info — IL12RB1
Entrez GeneID
3594Protein Accession#
P42701-1Gene Name
IL12RB1
Gene Alias
CD212, IL-12R-BETA1, IL12RB, MGC34454
Gene Description
interleukin 12 receptor, beta 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The lack of expression of this gene was found to result in the immunodeficiency of patients with severe mycobacterial and Salmonella infections. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
IL-12 receptor beta component|interleukin-12 receptor beta-1 chain
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Interactome
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Pathway
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Disease
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