DLL4 (Human) Recombinant Protein
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Specification
Product Description
Human DLL4 (Q9NR61, Ser27-Pro524) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Ser27-Pro524
Host
Human
Theoretical MW (kDa)
81.0999999999998
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 16.5 ng/mL, messured by ELISA at 1 ug/mL. The affinity constant of 0.48 uM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of- Human DLL4 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human DLL4 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human DLL4, hFc Tag at 1 ug/mL (100 uL/Well) on the plate. Dose response curve for Biotinylated Anti-DLL4 Antibody, hFc Tag with the EC50 of 16.5 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Human Notch 1, His Tag with an affinity constant of 0.48 uM as determined in SPR assay (Biacore T200). -
Gene Info — DLL4
Entrez GeneID
54567Protein Accession#
Q9NR61Gene Name
DLL4
Gene Alias
MGC126344, hdelta2
Gene Description
delta-like 4 (Drosophila)
Omim ID
605185Gene Ontology
HyperlinkGene Summary
This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. [provided by RefSeq
Other Designations
delta 4|delta ligand 4|delta-like 4 homolog|delta-like 4 protein|notch ligand DLL4|notch ligand delta-2
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