TFRC (Human) Recombinant Protein
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Specification
Product Description
Human TFRC (P02786-1, Cys89-Phe760) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Cys89-Phe760
Host
Human
Theoretical MW (kDa)
101.8
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 26.5 ng/mL, messured by ELISA at 2 ug/mL. The affinity constant of 0.1 uM as determined in SPR assay (Biacore T200).
Quality Control Testing
Tris-Bis PAGE
Tris-Bis PAGE
Human Transferrin R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human Transferrin R, hFc Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Anti-Transferrin R Antibody, hFc Tag with the EC50 of 26.5 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human Transferrin R, hFc Tag captured on CM5 Chip via Anti-Fc Antibody can bind Human Transferrin, His Tag with an affinity constant of 0.1 uM as determined in SPR assay (Biacore T200). -
Gene Info — TFRC
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Interactome
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Pathway
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Disease
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