SCG3 (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human SG3, His Tag at 0.2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-SG3 Antibody, hFc Tag with the EC50 of 20.6 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human SG3 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human SG3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human SCG3 (Q8WXD2-1, Phe20-Leu468) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Phe20-Leu468
Host
Human
Theoretical MW (kDa)
52.1
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 20.6 ng/mL, messured by ELISA at 0.2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human SG3 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human SG3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human SG3, His Tag at 0.2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-SG3 Antibody, hFc Tag with the EC50 of 20.6 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — SCG3
Entrez GeneID
29106Protein Accession#
Q8WXD2-1Gene Name
SCG3
Gene Alias
FLJ90833, SGIII
Gene Description
secretogranin III
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Granins may serve as precursors for biologically active peptides. Some granins have been shown to function as helper proteins in sorting and proteolytic processing of prohormones; however, the function of this protein is unknown. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
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Interactome
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Disease
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