KREMEN2 (Human) Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human Kremen-2, His Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-Kremen-2 Antibody, hFc Tag with the EC50 of 7.5 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human Kremen-2 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Kremen-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
-
Specification
Product Description
Human KREMEN2 (Q8NCW0-1, Gly26-Ala364) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Gly26-Ala364
Host
Human
Theoretical MW (kDa)
37.1
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 7.5 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human Kremen-2 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human Kremen-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
-
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human Kremen-2, His Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-Kremen-2 Antibody, hFc Tag with the EC50 of 7.5 ng/mL determined by ELISA.Functional Study
SDS-PAGE
-
Gene Info — KREMEN2
Entrez GeneID
79412Protein Accession#
Q8NCW0-1Gene Name
KREMEN2
Gene Alias
KRM2, MGC10791, MGC16709
Gene Description
kringle containing transmembrane protein 2
Omim ID
609899Gene Ontology
HyperlinkGene Summary
This gene encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein forms a ternary membrane complex with DKK1 and the WNT receptor lipoprotein receptor-related protein 6 (LRP6), and induces rapid endocytosis and removal of LRP6 from the plasma membrane. It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq
Other Designations
kringle-containing transmembrane protein 2
-
Interactome
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com