CD160 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human HVEM, His Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.93 ug/mL determined by ELISA.
SEC-HPLC
The purity of Human CD160 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD160 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human CD160 (O95971-1, Gly25-Leu158) partial recombinant protein with hFc tag at C-Terminus expressed in HEK293 cells.
Sequence
Gly25-Leu158
Host
Human
Theoretical MW (kDa)
41.3
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 0.93 ug/mL, messured by ELISA at 2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CD160 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD160 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human HVEM, His Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Human CD160, hFc Tag with the EC50 of 0.93 ug/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — CD160
Entrez GeneID
11126Protein Accession#
O95971-1Gene Name
CD160
Gene Alias
BY55, FLJ46513, NK1, NK28
Gene Description
CD160 molecule
Omim ID
604463Gene Ontology
HyperlinkGene Summary
CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. [provided by RefSeq
Other Designations
CD160 antigen|OTTHUMP00000015585|natural killer cell receptor, immunoglobulin superfamily member
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