GP6 (Human) Recombinant Protein
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Specification
Product Description
Human GP6 (Q9HCN6-1, Gln21-Lys267) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Gln21-Lys267
Host
Human
Theoretical MW (kDa)
28
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 4.2 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human GPVI is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human GPVI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human GPVI, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-GPVI Antibody, hFc Tag with the EC50 of 4.2 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — GP6
Entrez GeneID
51206Protein Accession#
Q9HCN6-1Gene Name
GP6
Gene Alias
GPIV, GPVI, MGC138168
Gene Description
glycoprotein VI (platelet)
Omim ID
605546Gene Ontology
HyperlinkGene Summary
Glycoprotein VI (GP6) is a 58-kD platelet membrane glycoprotein that plays a crucial role in the collagen-induced activation and aggregation of platelets. Upon injury to the vessel wall and subsequent damage to the endothelial lining, exposure of the subendothelial matrix to blood flow results in deposition of platelets. Collagen fibers are the most thrombogenic macromolecular components of the extracellular matrix, with collagen types I, III, and VI being the major forms found in blood vessels. Platelet interaction with collagen occurs as a 2-step procedure: (1) the initial adhesion to collagen is followed by (2) an activation step leading to platelet secretion, recruitment of additional platelets, and aggregation. In physiologic conditions, the resulting platelet plug is the initial hemostatic event limiting blood loss. However, exposure of collagen after rupture of atherosclerotic plaques is a major stimulus of thrombus formation associated with myocardial infarction or stroke (Jandrot-Perrus et al., 2000 [PubMed 10961879]).[supplied by OMIM
Other Designations
platelet collagen receptor|platelet glycoprotein VI
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Interactome
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