APOH (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human APOH, His Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 0.97 ug/mL determined by ELISA.
SEC-HPLC
The purity of Human APOH is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human APOH on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human APOH (P02749, Gly20-Cys3459) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Gly20-Cys345
Host
Human
Theoretical MW (kDa)
37.4
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 0.97 ug/mL, messured by ELISA at 2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human APOH is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human APOH on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human APOH, His Tag at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 0.97 ug/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — APOH
Entrez GeneID
350Protein Accession#
P02749Gene Name
APOH
Gene Alias
B2G1, BG
Gene Description
apolipoprotein H (beta-2-glycoprotein I)
Omim ID
138700Gene Ontology
HyperlinkGene Summary
Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections. [provided by RefSeq
Other Designations
apolipoprotein H|beta-2-glycoprotein I
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Interactome
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Disease
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