MMP8 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human MMP-8, His Tag at 5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 1.93 ug/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Anti-MMP-8 Antibody captured on CM5 Chip via Protein A can bind Human MMP-8, His Tag with an affinity constant of 1.14 uM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human MMP-8 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human MMP-8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human MMP8 (P22894, Phe21-Gly467) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Phe21-Gly467
Host
Human
Theoretical MW (kDa)
52.2
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 1.93 ug/mL, messured by ELISA at 5 ug/mL. The affinity constant of 1.14 uM as determined in SPR assay (Biacore T200). Measured by the ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is > 300 pmoles/min/ug (QC Test).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human MMP-8 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human MMP-8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human MMP-8, His Tag at 5 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 1.93 ug/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Anti-MMP-8 Antibody captured on CM5 Chip via Protein A can bind Human MMP-8, His Tag with an affinity constant of 1.14 uM as determined in SPR assay (Biacore T200). -
Gene Info — MMP8
Entrez GeneID
4317Protein Accession#
P22894Gene Name
MMP8
Gene Alias
CLG1, HNC, PMNL-CL
Gene Description
matrix metallopeptidase 8 (neutrophil collagenase)
Omim ID
120355Gene Ontology
HyperlinkGene Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq
Other Designations
PMNL collagenase|matrix metalloproteinase 8|matrix metalloproteinase 8 (neutrophil collagenase)|neutrophil collagenase
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Interactome
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Disease
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