DSG3 (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human DSG3, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-DSG3 Antibody, hFc Tag with the EC50 2.1 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human DSG3 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human DSG3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human DSG3 (P32926, Glu50-Arg615) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Glu50-Arg615
Host
Human
Theoretical MW (kDa)
64
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 2.1 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human DSG3 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human DSG3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human DSG3, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-DSG3 Antibody, hFc Tag with the EC50 2.1 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — DSG3
Entrez GeneID
1830Protein Accession#
P32926Gene Name
DSG3
Gene Alias
CDHF6, DKFZp686P23184, PVA
Gene Description
desmoglein 3 (pemphigus vulgaris antigen)
Omim ID
169615Gene Ontology
HyperlinkGene Summary
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This protein has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus vulgaris. [provided by RefSeq
Other Designations
130-kD pemphigus vulgaris antigen|desmoglein 3|pemphigus vulgaris antigen
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Interactome
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Disease
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