PLAU (Human) Recombinant Protein
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Specification
Product Description
Human PLAU (P00749-1, Ser21-Leu431) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
Sequence
Ser21-Leu431
Host
Human
Theoretical MW (kDa)
47.5
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 2.4 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human PLAU (pro form) is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human PLAU (pro form) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human PLAU, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 2.4 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — PLAU
Entrez GeneID
5328Protein Accession#
P00749-1Gene Name
PLAU
Gene Alias
ATF, UPA, URK, UROKINASE, u-PA
Gene Description
plasminogen activator, urokinase
Gene Ontology
HyperlinkGene Summary
This gene encodes a serine protease involved in degradation of the extracellular matrix and possibly tumor cell migration and proliferation. A specific polymorphism in this gene may be associated with late-onset Alzheimer's disease and also with decreased affinity for fibrin-binding. This protein converts plasminogen to plasmin by specific cleavage of an Arg-Val bond in plasminogen. Plasmin in turn cleaves this protein at a Lys-Ile bond to form a two-chain derivative in which a single disulfide bond connects the amino-terminal A-chain to the catalytically active, carboxy-terminal B-chain. This two-chain derivative is also called HMW-uPA (high molecular weight uPA). HMW-uPA can be further processed into LMW-uPA (low molecular weight uPA) by cleavage of chain A into a short chain A (A1) and an amino-terminal fragment. LMW-uPA is proteolytically active but does not bind to the uPA receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000019849|U-plasminogen activator|antagonist of uPA|plasminogen activator, urinary|urokinase plasminogen activator|urokinase-type plasminogen activator amino-terminal fragment
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