CD55 (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD55, His Tag at 0.2 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 14.3ng/mL determined by ELISA.
SEC-HPLC
The purity of Human CD55 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD55 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human CD55 (P08174-1, Asp35-Ser353) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
Sequence
Asp35-Ser353
Host
Human
Theoretical MW (kDa)
35.8
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 14.3 ng/mL, messured by ELISA at 0.2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CD55 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD55 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD55, His Tag at 0.2 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 14.3ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — CD55
Entrez GeneID
1604Protein Accession#
P08174-1Gene Name
CD55
Gene Alias
CR, CROM, DAF, TC
Gene Description
CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Omim ID
125240Gene Ontology
HyperlinkGene Summary
This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq
Other Designations
CD55 antigen|decay accelerating factor for complement
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Interactome
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Pathway
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Disease
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