L1CAM (Human) Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human L1CAM, His Tag at 0.2 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-L1CAM Antibody, hFc Tag with the EC50 of 8.7 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human L1CAM is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human L1CAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
-
Specification
Product Description
Human L1CAM (P32004-1, Ile20-Glu1120) partial recombinant protein with His tag at C-terminus expressed in HEK293 cells.
Sequence
Ile20-Glu1120
Host
Human
Theoretical MW (kDa)
124.6
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 8.7 ng/mL, messured by ELISA at 0.2 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human L1CAM is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human L1CAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
-
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human L1CAM, His Tag at 0.2 ug/mL (100 uL/well) on the plate. Dose response curve for Anti-L1CAM Antibody, hFc Tag with the EC50 of 8.7 ng/mL determined by ELISA.Functional Study
SDS-PAGE
-
Gene Info — L1CAM
Entrez GeneID
3897Protein Accession#
P32004-1Gene Name
L1CAM
Gene Alias
CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAML1, S10, SPG1
Gene Description
L1 cell adhesion molecule
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant. [provided by RefSeq
Other Designations
OTTHUMP00000025992|antigen identified by monoclonal antibody R1|neural cell adhesion molecule L1
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com