TNF (Bovine) Recombinant Protein
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Specification
Product Description
Bovine TNF partial recombinant protein expressed in Escherichia coli.
Sequence
MLRSSSQASSNKPVAHVVADINSPGQLRWWDSYANALMANGVKLEDNQLVVPADGLYLIYSQVLFRGQGCPSTPLFLTHTISRIAVSYQTKVNILSAIKSPCHRETPEWAEAKPWYEPIYQGGVFQLEKGDRLSAEINLPDYLDYAESGQVYFGIIAL.
Host
Escherichia coli
Theoretical MW (kDa)
17.5
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
chromatographic
Purity
> 90% as determined by SDS-PAGE.
Activity
ED50 < 15 ng/mL, measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
Storage Buffer
Solution (1 mg/mL) containing 1X PBS, pH 7.4, 10% glycerol.
Storage Instruction
Store at 4°C for one weeks and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — TNF
Entrez GeneID
280943Protein Accession#
Q06599Gene Name
TNF
Gene Alias
TNFa
Gene Description
tumor necrosis factor (TNF superfamily, member 2)
Gene Ontology
HyperlinkGene Summary
cachectin|tumor necrosis factor alpha|tumor necrosis factor
Other Designations
cachectin|tumor necrosis factor alpha|tumor necrosis factor, alpha (TNF superfamily, member 2)
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