FGF2 (Bovine) Recombinant Protein
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Specification
Product Description
Bovine FGF2 recombinant protein (containing 147 amino acid) expressed in?Escherichia coli.
Sequence
MPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYSSWYVALKRTGQYKLGPKTGPGQKAILFLPMSAKS
Host
Escherichia coli
Theoretical MW (kDa)
16.5
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
chromatography
Purity
> 97% as determined by (a) RP-HPLC.(b) SDS-PAGE.
Activity
ED50 < 0.1 ng/mL, measured by a cell proliferation assay using murine balb/c 3T3 cells, corresponding to a Specific Activity of > 1 x 107Units/mg.
Storage Buffer
Lyophilized from a solution containing 1X PBS, pH 7.4. Reconstitute the lyophilized powder in 1X PBS to 100 ug/mL.
Storage Instruction
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C.
Avoid repeated freeze/thaw cycles. -
Applications
Functional Study
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Gene Info — FGF2
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