Trimeric Spike (S) (SARS-CoV-2) Recombinant Protein
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Trimeric Spike (S) (SARS-CoV-2) Recombinant Protein was separated on SDS-PAGE under the reducing condition and visualized by Coomassie Blue staining.
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Specification
Product Description
Trimeric Spike (S) partial recombinant protein with polyhistidine tag at C-terminus expressed in HEK293 cells.
Sequence
SQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTILRSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPNGTGRMKQIEDKIENITSKIYNITNEIARIKKLIGNRT
Form
Liquid
Preparation Method
Mammalian cell (HEK 293) expression system
Purification
Ni-NTA chromatography
Purity
> 95% as analyzed by SDS-PAGE.
Endotoxin Level
< 0.1 EU/ ug of protein by the LAL method.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue.
Trimeric Spike (S) (SARS-CoV-2) Recombinant Protein was separated on SDS-PAGE under the reducing condition and visualized by Coomassie Blue staining.
Recommend Usage
Enzyme-linked Immunoabsorbent Assay
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4.
Storage Instruction
Store at -80°C.
Avoid repeated freeze/thaw cycles. -
Applications
Enzyme-linked Immunoabsorbent Assay
Binding assay of Trimeric Spike (S) (SARS-CoV-2) Recombinant Protein coating on ELISA plate is binding by ACE2-hFc and detected by anti-human IgG Fc-HRP.SDS-PAGE
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