Products

Product Description

Bovine TNF (Q06599, 78 a.a. - 234 a.a.) partial recombinant protein expressed in Escherichia coli.Bioactive Protein,Bioactive Proteins,Bioactive,Active,Functional Protein,Functional Proteins

Sequence

MLRSSSQASSNKPVAHVVADINSPGQLRWWDSYANALMANGVKLEDNQLVVPADGLYLIYSQVLFRGQGCPSTPLFLTHTISRIAVSYQTKVNILSAIKSPCHRETPEWAEAKPWYEPIYQGGVFQLEKGDRLSAEINLPDYLDYAESGQVYFGIIAL

Host

Escherichia coli

Theoretical MW (kDa)

17.5

Form

Liquid

Preparation Method

Escherichia coli expression system

Purity

> 90% as analyzed by SDS-PAGE.

Endotoxin Level

< 1 EU/ug of protein by the LAL method.

Activity

ED₅₀ < 15 ng/mL, measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.

Quality Control Testing

SDS-PAGE Stained with Coomassie Blue.

SDS-PAGE analysis of TNF (Bovine) Recombinant Protein.

Recommend Usage

Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.

Storage Buffer

In PBS, pH 7.4 (10% glycerol)

Storage Instruction

Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.

Note

Result of activity analysis