Ccl2 (Mouse) Recombinant Protein
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Specification
Product Description
Mouse Ccl2 (Q5SVU3, 24 a.a. - 96 a.a.) partial recombinant protein expressed in HEK293 cells.
Sequence
QPDAVNAPLTCCYSFTSKMIPMSRLESYKRITSSRCPKEAVVFVTKLKREVCADPKKEWVQTYIKNLDRNQMR
Host
Human
Theoretical MW (kDa)
~ 8
Form
Lyophilized
Preparation Method
Mammalian cell (HEK 293) expression system
Purity
> 98% as analyzed by SDS-PAGE.
Endotoxin Level
< 0.2 EU/ ug of protein (gel clotting method)
Activity
EC50 of mouse MCP-1/CCL2 < 0.3 ug/mL, measured by Ca2+ mobilization assay in CHO-K1/Galpha15/mCCR2 cells (human Galpha15 and mouse CCR2 stably expressed in CHO-K1 cells).
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O or PBS up to 100 ug/mL.
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Ccl2
Entrez GeneID
20296Protein Accession#
Q5SVU3Gene Name
Ccl2
Gene Alias
AI323594, HC11, JE, MCAF, MCP-1, MCP1, SMC-CF, Scya2, Sigje
Gene Description
chemokine (C-C motif) ligand 2
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000000786|monocyte chemoattractant protein-1|monocyte chemotactic protein|small inducible cytokine A2
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