Il12a and Il12b (Rat) Recombinant Protein
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Specification
Product Description
Rat Il12a (Q9R103, 23 a.a. - 215 a.a.) partial recombinant protein with His tag and Il12b (E9PU71, 23 a.a. - 335 a.a.) partial recombinant protein expressed in CHO cells.
Sequence
RVIPVSGPAKCLNQSQNLLKTTDDMVRTAREKLKHYSCTAGDIDHEDITRDKTSTLEACLPLELHKNESCLATKETSSIIRGSCLPPQKTSLMMTLCLGSIYEDLKMYQSEFQAINAALQSHNHQQITLDRNMLMAIDELMRSLNHSGETLHQKAPMGEADPYRVKMKLCILLHAFSTRVMTINRVMNYLSSS MWELEKDVYVVEVDWRPDAPGETVTLTCDSPEEDDITWTSDQRRGVIGSGKTLTITVREFLDAGQYTCHRGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVHRNTDLKFNIKSSSSSPESRAVTCGRASLSAEKVTLNQRDYEKYSVACQEDVTCPTAEETLPIELVVEAQQQNKYENYSTSFFIRDIIKPDPPKNLQVKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKTKETEEECNQKGAFLVEKTSAEVQCKGANICVQAQDRYYNSSCSKWTCVPCRGRS
Host
Mammals
Theoretical MW (kDa)
26 ~ 28, 42 ~ 50
Form
Lyophilized
Preparation Method
Mammalian cell (CHO) expression system
Purity
> 95% as analyzed by SDS-PAGE.
Endotoxin Level
< 0.2 EU/ ug of protein (gel clotting method)
Activity
ED50 < 0.1 ng/mL, measured in a cell proliferation assay using 2D6 cells.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS. Reconstitute the lyophilized powder in ddH2O or PBS up to 100 ug/mL.
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Il12b
Entrez GeneID
64546Protein Accession#
Q9R103,E9PU71Gene Name
Il12b
Gene Alias
Il12
Gene Description
interleukin 12b
Gene Ontology
HyperlinkOther Designations
interleukin-12 p40
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Gene Info — Il12a
Entrez GeneID
84405Protein Accession#
Q9R103,E9PU71Gene Name
Il12a
Gene Alias
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Gene Description
interleukin 12a
Gene Ontology
HyperlinkOther Designations
interleukin 12 p35 subunit
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