Products

Product Description

Recombinant Streptococcus pyogenes Cas9 nuclease expressed in an Escherichia coli with a double-ended nuclear localization signal (NLS).

Host

Escherichia coli

Theoretical MW (kDa)

~160 kDa

Form

Liquid

Preparation Method

Escherichia coli expression system

Purity

≥ 95% as analyzed by SDS-PAGE
≥ 90% as analyzed by SEC-HPLC

Endotoxin Level

≤ 10 EU/mg as analyzed by gel clotting method

Quality Control Testing

Cleavage assay

A 20 uL reaction in 1xCas9 Nuclease Reaction Buffer containing 160 ng linearized plasmid, 100 ng gRNA, and 50 ng Ultra eSpCas9-2NLS-Research for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.

Recommend Usage

CRISPR Genomic editing
The optimal working dilution should be determined by the end user.

Storage Buffer

In 25 mM Tris, 300 mM NaCl, 0.1 mM EDTA, pH8.0 (50% glycerol).

Storage Instruction

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.