T7 Endonuclease I
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Specification
Product Description
Fusion protein T7 Endonuclease I expressed in an Escherichia coli.
Host
Escherichia coli
Form
Liquid
Preparation Method
Escherichia coli expression system
Quality Control Testing
Cleavage assay
The T7 Endonuclease I assay to detect the cleavage efficiency of control HPRT gRNA with Cas9 protein.
Recommend Usage
CRISPR Genomic editing
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris-HCl, 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.15% Triton X-100, pH7.5 (50% glycerol).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
CRISPR Genomic Editing
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