Products

Product Description

Fusion protein T7 Endonuclease I expressed in an Escherichia coli.

Host

Escherichia coli

Form

Liquid

Preparation Method

Escherichia coli expression system

Quality Control Testing

Cleavage assay

The T7 Endonuclease I assay to detect the cleavage efficiency of control HPRT gRNA with Cas9 protein.

Recommend Usage

CRISPR Genomic editing
The optimal working dilution should be determined by the end user.

Storage Buffer

In 20 mM Tris-HCl, 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.15% Triton X-100, pH7.5 (50% glycerol).

Storage Instruction

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.