IL21 (Canine) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
IL21 (Canine) Recombinant Protein (Cat # P6984) stimulates IFN-g secretion of the NK-92 human natural killer cells. The ED50 range ≤ 2 ng/mL.
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Specification
Product Description
Canine IL21 (NP_001003347.1, 18 a.a. - 146 a.a.) partial recombinant protein expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
15
Form
Liquid
Preparation Method
Escherichia coli expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method).
Activity
The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤ 2 ng/mL.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Recommend Usage
Bioactivity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4.
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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Gene Info — IL21
Entrez GeneID
442935Protein Accession#
NP_001003347.1Gene Name
IL21
Gene Alias
IL-21
Gene Description
interleukin 21
Gene Ontology
HyperlinkOther Designations
interleukin-21
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