N (SARS-CoV-2) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
ELISA plate was coated by N protein, 100 uL/well, at various concentrations. The direct ELISA analysis was performed by loading 100 uL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hour at 37°C, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2 M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.
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Specification
Product Description
SARS-CoV-2 N (QHD43423.2) full-length recombinant protein with His tag at C-terminal expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
47.08
Specificity
No non-specific binding to normal human serum was identified so far.
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Ni column
Purity
> 95% by SDS-PAGE.
Quality Control Testing
DTT-reducing SDS-PAGE Result
Recommend Usage
Antigen
ELISA
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM Tris, pH 8.5.
Storage Instruction
This protein is not stable at 4°C in liquid. For long term storage store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
ELISA plate was coated by N protein, 100 uL/well, at various concentrations. The direct ELISA analysis was performed by loading 100 uL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hour at 37°C, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2 M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.SDS-PAGE
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