V5 tag monoclonal antibody, clone V5.E10
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of V5 tag.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to V5 tag.
Host
Mouse
Specificity
Recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins.
Form
Liquid
Purification
Protein A purification
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
Western Blot (1:1000-1:3000)
Immunohistochemistry (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2). Blotting with V5 tag monoclonal antibody, clone V5.E10 (Cat # MAB9755) at 1:000 dilution.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot
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