V5 tag monoclonal antibody, clone V5.E10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of V5 tag monoclonal antibody, clone V5.E10 (Cat # MAB8756) in 293 cells.
Lane 1 : 293 cells untransfected control.
Lane 2 : 293 cells tenasfected with lacZ-V5-tagged.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant V5 tag.
Immunogen
Recombinant protein corresponding to V5 tag.
Sequence
GKPIPNPLLGLDST
Host
Mouse
Specificity
This antibody detects (GKPIPNPLLGLDST) tag fusion protein.
Form
Liquid
Purification
Protein A purification
Concentration
1 ug/uL
Recommend Usage
ELISA (1:5000-10000)
Western Blot (1:1000)
IHC (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of V5 tag monoclonal antibody, clone V5.E10 (Cat # MAB8756) in 293 cells.
Lane 1 : 293 cells untransfected control.
Lane 2 : 293 cells tenasfected with lacZ-V5-tagged.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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