SLA (haploid sperm cell-specific antigen) monoclonal antibody, clone TRA54
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Specification
Product Description
Rat monoclonal antibody raised against SLA (haploid sperm cell-specific antigen).
Immunogen
The hybridoma producing TRA54 was obtained from the immunaization of a rat with cell lysates of adult mouse testis.
Host
Rat
Reactivity
Mouse
Form
Liquid
Isotype
IgM
Recommend Usage
Immunohistochemistry
Western Blot (~1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot analysis of various mouse tissues with SLA (haploid sperm cell-specific antigen) monoclonal antibody, clone TRA54 (Cat # MAB7952).Immunohistochemistry
Immunohistochemical staining of mouse testis with SLA (haploid sperm cell-specific antigen) monoclonal antibody, clone TRA54 (Cat # MAB7952). -
Publication Reference
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Spatiotemporal expression of UPK3B and its promoter activity during embryogenesis and spermatogenesis.
Kuriyama S, Tamiya Y, Tanaka M.
Histochemistry and Cell Biology 2017 Jan; 147(1):17.
Application:IF, Mouse, Mouse testis.
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Identification and characterization of an antigen recognized by monoclonal antibody TRA 54 in mouse epididymis and vas deferens.
Arroteia KF, Joazeiro PP, Yamada AT, Tanaka H, Nishimune Y, Pereira LA.
Journal of Andrology 2004 Nov; 25(6):914.
Application:IHC, WB-Ti, Mouse, Testis, Epididymis, Sperm, Brain, Liver, Kidney, Spleen.
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Intercellular organelle traffic through cytoplasmic bridges in early spermatids of the rat: mechanisms of haploid gene product sharing.
Ventela S, Toppari J, Parvinen M.
Molecular Biology of the Cell 2003 Jul; 14(7):2768.
Application:IF, IEM, Rat, Spermatocytes.
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Characterization and expression of a stage specific antigen by monoclonal antibody TRA 54 in testicular germ cells.
Pereira LA, Tanaka H, Nagata Y, Sawada K, Mori H, Chimelli LM, Nishimune Y.
Journal of Andrology 1998 Feb; 21(1):34.
Application:IHC-P, WB-Ti, Mouse, Mouse testes, testicular germ cells.
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Spatiotemporal expression of UPK3B and its promoter activity during embryogenesis and spermatogenesis.
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