CDC27 monoclonal antibody, clone AF3.1
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of CDC27.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 814-823 of human CDC27.
Host
Mouse
Reactivity
Bovine, Dog, Human, Mouse, Rat, Xenopus
Specificity
Cdc27 (AF3.1). In WB detects a band of approximately 97 KDa.
Form
Liquid
Isotype
IgG2a
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS, pH 7.4
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDC27
Entrez GeneID
996Gene Name
CDC27
Gene Alias
APC3, CDC27Hs, D0S1430E, D17S978E, HNUC
Gene Description
cell division cycle 27 homolog (S. cerevisiae)
Omim ID
116946Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
anaphase-promoting complex, protein 3|cell division cycle protein 27|nuc2 homolog
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Interactome
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Pathway
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Publication Reference
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Expression of the CDH1-associated form of the anaphase-promoting complex in postmitotic neurons.
Gieffers C, Peters BH, Kramer ER, Dotti CG, Peters JM.
PNAS 1999 Sep; 96(20):11317.
Application：IHC, IF, WB-Ce, WB-Ti, Human, Mouse, Rat, HeLa cells, Mouse brains, Rat hippocampal neurons.
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The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor.
Yamano H, Tsurumi C, Gannon J, Hunt T.
The EMBO Journal 1998 Oct; 17(19):5670.
Application：IP, Xenopus, Egg.
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The ubiquitin pathway for protein degradation.
Hershko A.
Trends in Biochemical Sciences 1991 Jul; 16(7):265.
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Expression of the CDH1-associated form of the anaphase-promoting complex in postmitotic neurons.
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