LYS20/LYS21 monoclonal antibody, clone 31F5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blots of whole yeast protein extracts with LYS20/LYS21 monoclonal antibody, clone 31F5 (Cat # MAB5385).
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Specification
Product Description
Mouse monoclonal antibody raised against LYS20/LYS21.
Immunogen
Nuclear protein from yeast.
Host
Mouse
Theoretical MW (kDa)
47.1, 48.6
Reactivity
Yeast
Form
Liquid
Isotype
IgG1
Recommend Usage
Western Blot (1:10000)
Immunofluorescence (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In tissue culture supernatant (5 mM sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blots of whole yeast protein extracts with LYS20/LYS21 monoclonal antibody, clone 31F5 (Cat # MAB5385).Immunohistochemistry
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — LYS20
Entrez GeneID
851346Protein Accession#
YDL182W (Gene ID : 851346);YDL131W (Gene ID : 851425)Gene Name
LYS20
Gene Alias
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Gene Description
Homocitrate synthase isozyme, catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; highly similar to the other isozyme, Lys21p
Gene Ontology
HyperlinkGene Summary
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Other Designations
Lys20p
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Gene Info — LYS21
Entrez GeneID
851425Protein Accession#
YDL182W (Gene ID : 851346);YDL131W (Gene ID : 851425)Gene Name
LYS21
Gene Alias
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Gene Description
Homocitrate synthase isozyme, catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; highly similar to the other isozyme, Lys20p
Gene Ontology
HyperlinkGene Summary
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Other Designations
Lys21p
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Publication Reference
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Homocitrate synthase is located in the nucleus in the yeast Saccharomyces cerevisiae.
Chen S, Brockenbrough JS, Dove JE, Aris JP.
The Journal of Biological Chemistry 1997 Apr; 272(16):10839.
Application：IF, WB, Yeast, Yeast cells.
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Homocitrate synthase is located in the nucleus in the yeast Saccharomyces cerevisiae.
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