CD5 monoclonal antibody, clone CRIS1 (FITC)
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Specification
Product Description
Mouse monoclonal antibody raised against native CD5.
Immunogen
Native purified CD5 from human stimulated leukocytes.
Host
Mouse
Theoretical MW (kDa)
67
Reactivity
Human
Specificity
This antibody reacts with the cell surface glycoprotein CD5, a 67KDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
Form
Liquid
Conjugation
FITC
Isotype
IgG2a
Recommend Usage
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Frozen sections)
Immunoprecipitation
Flow Cytometry
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Gene Info — CD5
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Interactome
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Pathway
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Disease
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Publication Reference
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Conformation of the hypervariable region L3 without the key proline residue.
Guarne A, Bravo J, Calvo J, Lozano F, Vives J, Fita I.
Protein Science: a Publication of the Protein Society 1996 Jan; 5(1):167.
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Intracellular events involved in CD5-induced human T cell activation and proliferation.
Alberola-Ila J, Places L, Cantrell DA, Vives J, Lozano F.
Journal of Immunology (Baltimore, Md. : 1950) 1992 Mar; 148(5):1287.
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Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis.
Arrizabalaga P, Mirapeix E, Darnell A, Torras A, Revert L.
Nephron 1989 Jan; 53(1):41.
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Conformation of the hypervariable region L3 without the key proline residue.
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