Neurofilament medium protein monoclonal antibody, clone NF-09
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blotting analysis of Neurofilament medium protein in porcine brain lysate (reducing conditions) by Neurofilament medium protein monoclonal antibody, clone NF-09 (Cat # MAB3872).
Immunofluorescence
Immunofluorescence staining of neurofilament medium protein in murine Neuro2A cells by Neurofilament medium protein monoclonal antibody, clone NF-09 (Cat # MAB3872) conjugated with Dyomics 547 (red). DNA stained by Hoechst (blue).
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Specification
Product Description
Mouse monoclonal antibody raised against native Neurofilament medium protein.
Immunogen
Native purified from porcine brain cold stable proteins after depolymerization of microtubules.
Host
Mouse
Theoretical MW (kDa)
160
Reactivity
Mammals, Pig
Specificity
This antibody reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 KDa) of various species.
Form
Liquid
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blotting analysis of Neurofilament medium protein in porcine brain lysate (reducing conditions) by Neurofilament medium protein monoclonal antibody, clone NF-09 (Cat # MAB3872).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunofluorescence staining of neurofilament medium protein in murine Neuro2A cells by Neurofilament medium protein monoclonal antibody, clone NF-09 (Cat # MAB3872) conjugated with Dyomics 547 (red). DNA stained by Hoechst (blue). -
Publication Reference
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Regulation between O-GlcNAcylation and phosphorylation of neurofilament-M and their dysregulation in Alzheimer disease.
Deng Y, Li B, Liu F, Iqbal K, Grundke-Iqbal I, Brandt R, Gong CX.
FASEB Journal 2008 Jan; 22(1):138.
Application：IF, IHC, WB-Ti, Rat, Rat brain.
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The neurofilament middle molecular mass subunit carboxyl-terminal tail domains is essential for the radial growth and cytoskeletal architecture of axons but not for regulating neurofilament transport rate.
Rao MV, Campbell J, Yuan A, Kumar A, Gotow T, Uchiyama Y, Nixon RA.
The Journal of Cell Biology 2003 Dec; 163(5):1021.
Application：WB-Ti, Mouse, Sciatic, Optic nerve.
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Monoclonal antibody NF-09 specific for neurofilament protein NF-M.
Dráberová E, Sulimenko V, Kukharskyy V, Dráber P.
Folia Biologica 1999 Mar; 45(4):163.
Application：IF, WB-Ti, Mouse, Pig, Brain, P19 cells.
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Regulation between O-GlcNAcylation and phosphorylation of neurofilament-M and their dysregulation in Alzheimer disease.
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