CD47 monoclonal antibody, clone MEM-122
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More Files
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Flow Cytometry
Surface staining of lysed and washed porcine peripheral blood with CD47 monoclonal antibody, clone MEM-122 (Cat # MAB3839) (detection by anti-mouse IgM FITC).
Panel A - porcine PBMC stained with Isotype mouse IgM control.
Panel B, C, D - porcine PBMC sample stained with CD47 monoclonal antibody, clone MEM-122 (Cat # MAB3839). Cells in the granulocyte gate were used for analysis.
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Specification
Product Description
Mouse monoclonal antibody raised against native CD47.
Immunogen
Native purified CD47 from African Green Monkey COS-7 cells.
Host
Mouse
Theoretical MW (kDa)
50-55
Reactivity
Human
Specificity
This antibody reacts with CD47 (Integrin Associated Protein), a 50-55 KDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergenefamily) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.
Form
Liquid
Concentration
1 mg/mL
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Frozen sections)
Flow Cytometry
Surface staining of lysed and washed porcine peripheral blood with CD47 monoclonal antibody, clone MEM-122 (Cat # MAB3839) (detection by anti-mouse IgM FITC).
Panel A - porcine PBMC stained with Isotype mouse IgM control.
Panel B, C, D - porcine PBMC sample stained with CD47 monoclonal antibody, clone MEM-122 (Cat # MAB3839). Cells in the granulocyte gate were used for analysis. -
Gene Info — CD47
Entrez GeneID
961Gene Name
CD47
Gene Alias
IAP, MER6, OA3
Gene Description
CD47 molecule
Omim ID
601028Gene Ontology
HyperlinkGene Summary
This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD47 antigen|CD47 antigen (Rh-related antigen, integrin-associated signal transducer)|CD47 glycoprotein|Rh-related antigen|antigen identified by monoclonal antibody 1D8|antigenic surface determinant protein OA3|integrin associated protein|integrin-associa
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Interactome
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Pathway
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Publication Reference
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CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model.
Orazizadeh M, Lee HS, Groenendijk B, Sadler SJ, Wright MO, Lindberg FP, Salter DM.
Arthritis Research & Therapy 2008 Jan; 10(1):R4.
Application：Func, IHC, WB, Human, Human articular cartilage, normal and osteoarthritic chondrocytes.
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Blockade of thrombospondin-1-CD47 interactions prevents necrosis of full thickness skin grafts.
Isenberg JS, Pappan LK, Romeo MJ, Abu-Asab M, Tsokos M, Wink DA, Frazier WA, Roberts DD.
Annals of Surgery 2008 Jan; 247(1):180.
Application：WB-Ce, Human, HUVECs.
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Role for CD47-SIRPalpha signaling in xenograft rejection by macrophages.
Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H.
PNAS 2007 Mar; 104(12):5062.
Application：Flow cyt, IF, Human, PBMC, Porcine LCL, pKS336-transfected porcine LCL, pKS336-human CD47-transfected porcine LCL cells.
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CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model.
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