Phosphotyrosine monoclonal antibody, clone P-Tyr-01
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Specification
Product Description
Mouse monoclonal antibody raised against Phosphotyrosine.
Immunogen
Phosphotyrosine conjugated with BSA.
Host
Mouse
Specificity
This antibody detects tyrosine phosphorylation in activated cells.
Form
Liquid
Concentration
1 mg/mL
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
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Publication Reference
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Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation.
Meraner P, Horejsi V, Wolpl A, Fischer GF, Stingl G, Maurer D.
Journal of Immunology 2007 Feb; 178(4):2262.
Application:WB, Human, Human monocyte-derived DCs .
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Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation.
Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, Drbal K, Kuramitsu Y, Kornacker B, Horejsi V, Schraven B.
The Journal of Experimental Medicine 2000 May; 19(19):1591.
Application:WB-Ce, WB-Tr, Human, Monkey, Jurkat, Raji, COS cells.
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T cell receptor signalling results in rapid tyrosine phosphorylation of the linker protein LAT present in detergent-resistant membrane microdomains.
Brdiĉka T, Cerný J, Horejŝí V.
Biochemical and Biophysical Research Communications 1998 Jul; 248(2):356.
Application:WB-Ce, Human, Jurkat cells.
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Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation.
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