Blood Group Lewis a monoclonal antibody, clone 7LE
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Specification
Product Description
Mouse monoclonal antibody raised against native Blood Group Lewis a.
Immunogen
Native purified human Blood Group Lewis a.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes Lewisa blood group antigen, a carbohydrate determinantcarried on both glycolipids and glycoproteins, expressed in colonic epithelial cells. Lewisamay be useful for detection of gastrointestinal, pancreatic and colorectal tumors.
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry staining of human colorectal adenocarcinoma (paraffin-embedded sections) with Blood Group Lewis a monoclonal antibody, clone 7LE (Cat # MAB3592).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Alteration of sialyl Lewis epitope expression in pterygium.
Creuzot-Garcher C, Guerzider V, Assem M, Bron AM, Delannoy P, Bara J.
Investigative Ophthalmology & Visual Science 1999 Jul; 40(8):1631.
Application:H&E/Others, Human, Goblet cells.
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Lewis antigen alterations in gastric cancer precursors.
Torrado J, Correa P, Ruiz B, Bernardi P, Zavala D, Bara J.
Gastroenterology 1992 Feb; 102(2):424.
Application:IHC, Human, Gastric cancer.
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Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens.
Bara J, Gautier R, Le Pendu J, Oriol R.
The Biochemical Journal 1988 Aug; 254(1):185.
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Alteration of sialyl Lewis epitope expression in pterygium.
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