RPS27A monoclonal antibody, clone Ubi-1
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More Files
- More Functions
Western Blot
Western blot analysis of RPS27A using RPS27A monoclonal antibody, clone Ubi-1 (Cat # MAB2414). Lane 1 is mono-ubiquitin and polyubiquitin chains. Lane 2 is monoubiquitin only (overloaded). Lanes 3, 4 and 5 are 0.1 mg rat cerebellum, cortex, and brain stem respectively. 20% gels with PVDF blots (Nitrocellulose works but not as well).
Immunohistochemistry
Immunohistochemical staining of RPS27A in hippocampal tissue from an Alzheimer patient, using RPS27A monoclonal antibody, clone Ubi-1 (Cat # MAB2414).
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Specification
Product Description
Mouse monoclonal antibody raised against RPS27A.
Immunogen
Purified ubiquitin conjugated with KLH with glutaraldehyde.
Host
Mouse
Reactivity
Bovine, Chicken, Fruit fly, Human, Mouse, Nematoda, Plants
Specificity
This antibody specifically recognizes ubiquitinated cytoplasmic inclusion bodies.
Form
Liquid
Isotype
IgG1, kappa
Quality Control Testing
Antibody Reactive Against UBC.
Recommend Usage
Immunofluorescence (1:500)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In buffer containing 5 mM sodium azide
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of RPS27A using RPS27A monoclonal antibody, clone Ubi-1 (Cat # MAB2414). Lane 1 is mono-ubiquitin and polyubiquitin chains. Lane 2 is monoubiquitin only (overloaded). Lanes 3, 4 and 5 are 0.1 mg rat cerebellum, cortex, and brain stem respectively. 20% gels with PVDF blots (Nitrocellulose works but not as well).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunohistochemistry
Immunohistochemical staining of RPS27A in hippocampal tissue from an Alzheimer patient, using RPS27A monoclonal antibody, clone Ubi-1 (Cat # MAB2414).Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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COP9 signalosome- and 26S proteasome-dependent regulation of SCFTIR1 accumulation in Arabidopsis.
Stuttmann J, Lechner E, Guerois R, Parker JE, Nussaume L, Genschik P, Noel LD.
The Journal of Biological Chemistry 2009 Jan; 284(12):7920.
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Suppression of the deubiquitinating enzyme USP5 causes the accumulation of unanchored polyubiquitin and the activation of p53.
Dayal S, Sparks A, Jacob J, Allende-Vega N, Lane DP, Saville MK.
The Journal of Biological Chemistry 2009 Feb; 284(8):5030.
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A drosophila model for amyotrophic lateral sclerosis reveals motor neuron damage by human SOD1.
Watson MR, Lagow RD, Xu K, Zhang B, Bonini NM.
The Journal of Biological Chemistry 2008 Jul; 283(36):24972.
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Alzheimer disease-specific conformation of hyperphosphorylated paired helical filament-Tau is polyubiquitinated through Lys-48, Lys-11, and Lys-6 ubiquitin conjugation.
Cripps D, Thomas SN, Jeng Y, Yang F, Davies P, Yang AJ.
The Journal of Biological Chemistry 2006 Apr; 281(16):10825.
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Identification of metabolic enzymes in renal cell carcinoma utilizing PROTEOMEX analyses.
Lichtenfels R, Kellner R, Atkins D, Bukur J, Ackermann A, Beck J, Brenner W, Melchior S, Seliger B.
Biochimica et Biophysica Acta 2003 Mar; 1646(1-2):21.
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COP9 signalosome- and 26S proteasome-dependent regulation of SCFTIR1 accumulation in Arabidopsis.
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