VTG monoclonal antibody, clone 5A4
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Specification
Product Description
Mouse monoclonal antibody raised against native vitellogenin.
Immunogen
Native purified Red Sea Bream vitellogenin.
Host
Mouse
Reactivity
Fish
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Native Purified Protein.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.25-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.1% BSA, 0.1% proclin)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of extract from red sea bream (lane 1) , carp (lane 2) , mummichog (lane 3) and medaka (lane 4) , using Vitellogenin monoclonal antibody, clone 5A4 (Cat # MAB1730) .Enzyme-linked Immunoabsorbent Assay
Standard curve for red sea bream, carp, mummichog and medaka ELISA, using Vitellogenin monoclonal antibody, clone 5A4 (Cat # MAB1730) . -
Publication Reference
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Transition of serum vitellogenin cycle in Sakhalin taimen (Hucho perryi).
Hiramatsu N, Shimizu M, Fukada H, Kitamura M, Ura K, Fuda H, Hara A.
Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology 1997 OCt; 118(2):149.
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Relationship between vitellogenin and its related egg yolk proteins in Sakhalin taimen (Hucho perryi).
Hiramatsu N, Hara A.
Comparative Biochemistry and Physiology. Part A, Physiology 1996 Nov; 115(3):243.
Application:WB-Re, Recombinant protein.
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Transition of serum vitellogenin cycle in Sakhalin taimen (Hucho perryi).
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