ARHGEF4 monoclonal antibody, clone ARHGEF-08
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) HEK293 cells, (2) HEK293-ARHGEF4 transfectants.
Flow Cytometry
Flow cytometric analysis (intracellular staining) of ARHGEF4 in transfected HEK-293 cells using anti-ARHGEF4 PE.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human ARHGEF4.
Immunogen
Recombinant protein corresponding to amino acids 143-271 of human ARHGEF4.
Host
Mouse
Reactivity
Human
Specificity
This antibody recognizes human intracellular protein ARHGEF4/ASEF1, approximately 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42.
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Flow Cytometry (intracellular staining)
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (15 mM sodium azide).
Storage Instruction
Store at 4°C. Do not freeze.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) HEK293 cells, (2) HEK293-ARHGEF4 transfectants.Flow Cytometry
Flow cytometric analysis (intracellular staining) of ARHGEF4 in transfected HEK-293 cells using anti-ARHGEF4 PE. -
Gene Info — ARHGEF4
Entrez GeneID
50649Gene Name
ARHGEF4
Gene Alias
ASEF, ASEF1, GEF4, STM6
Gene Description
Rho guanine nucleotide exchange factor (GEF) 4
Omim ID
605216Gene Ontology
HyperlinkGene Summary
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. This protein is similar to rat collybistin protein. Alternative splicing of this gene generates two transcript variants which encode different isoforms. Also there is possibility for the usage of multiple polyadenylation sites for this gene. [provided by RefSeq
Other Designations
APC-stimulated guanine nucleotide exchange factor|Rho guanine nucleotide exchange factor 4
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Interactome
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Pathway
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Publication Reference
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Structural basis for the recognition of Asef by adenomatous polyposis coli.
Zhenyi Zhang, Leyi Chen, Lei Gao, Kui Lin, Liang Zhu, Yang Lu, Xiaoshan Shi, Yuan Gao, Jing Zhou, Ping Xu, Jian Zhang, Geng Wu.
Cell Research 2012 Feb; 22(2):372.
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The RAC specific guanine nucleotide exchange factor Asef functions downstream from TEL-AML1 to promote leukaemic transformation.
Ruth Lyons, Owen Williams, Michelle Morrow, Neil Sebire, Mike Hubank, John Anderson.
Leukemia Research 2010 Jan; 34(1):109.
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Adenomatous polyposis coli and Asef function downstream of hepatocyte growth factor and phosphatidylinositol 3-kinase.
Yoshihiro Kawasaki, Shinnosuke Tsuji, Masaki Sagara, Kanae Echizen, Yoko Shibata, and Tetsu Akiyama.
The Journal of Biological Chemistry 2009 Aug; 284(33):22436.
Application：IF, IP-WB, Human, HeLa, MDA-MB-157, H1666, Caco-2, DLD-1 cells.
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Structural basis for the recognition of Asef by adenomatous polyposis coli.
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