GFAP monoclonal antibody, clone G-A-5
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Specification
Product Description
Mouse monoclonal antibody raised against native GFAP.
Immunogen
Native purified pig GFAP.
Host
Mouse
Theoretical MW (kDa)
49, 50, 49.5
Reactivity
Human, Mouse, Pig, Rat
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG1
Recommend Usage
Western Blot (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.4-1 ug/mL)
Immunohistochemistry (Frozen sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 1.2% sodium acetate (2 mg BSA, 0.01 mg sodium azide)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 1 mL of 1.2% sodium acetate or neutral PBS and concentration will be 100 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
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Gene Info — GFAP
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Publication Reference
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Infantile Alexander disease: spectrum of GFAP mutations and genotype-phenotype correlation.
Rodriguez D, Gauthier F, Bertini E, Bugiani M, Brenner M, N'guyen S, Goizet C, Gelot A, Surtees R, Pedespan JM, Hernandorena X, Troncoso M, Uziel G, Messing A, Ponsot G, Pham-Dinh D, Dautigny A, Boespflug-Tanguy O.
American Journal of Human Genetics 2001 Nov; 69(5):1134.
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Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.
Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE, Messing A.
Nature Genetics 2001 Jan; 27(1):117.
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Infantile Alexander disease: spectrum of GFAP mutations and genotype-phenotype correlation.
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