CA9 monoclonal antibody, clone CA9/781
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human CA9.
Immunogen
Recombinant protein corresponding to full length human CA9.
Host
Mouse
Theoretical MW (kDa)
55
Reactivity
Human
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG2b, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western Blot analysis of HCT-116 cell lysate with CA9 monoclonal antibody, clone CA9/781 (Cat # MAB13458).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human renal cell carcinoma with CA9 monoclonal antibody, clone CA9/781 (Cat # MAB13458).Immunofluorescence
Flow Cytometry
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Gene Info — CA9
Entrez GeneID
768Protein Accession#
Q16790Gene Name
CA9
Gene Alias
CAIX, MN
Gene Description
carbonic anhydrase IX
Omim ID
603179Gene Ontology
HyperlinkGene Summary
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq
Other Designations
OTTHUMP00000022773|RCC-associated protein G250|carbonic dehydratase
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