CDK2 monoclonal antibody, clone AN4.3
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Specification
Product Description
Mouse monoclonal antibody raised against human CDK2.
Immunogen
Recombinant protein corresponding to human CDK2.
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5 ug/mL)
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.1 M Tris-glycine, 0.15 M NaCl, pH 7.4 (0.05% sodium azide) and before the addition of glycerol to 30%.
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta with CDK2 monoclonal antibody, clone AN4.3 (Cat # MAB12550) at 5 ug/mL working concentration.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDK2
Entrez GeneID
1017Gene Name
CDK2
Gene Alias
p33(CDK2)
Gene Description
cyclin-dependent kinase 2
Omim ID
116953Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq
Other Designations
cdc2-related protein kinase|cell devision kinase 2|p33 protein kinase
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